We observed 'protein ubiquitination' and 'apoptosis signaling' pathways were both enriched in the two breast cancer models while 'IGF signaling' and 'cell motility' pathways were enriched in BT474 and 'amino acid metabolism' were enriched in the SKBR3 cell line. Pathway inference tools were used to interpret these proteins in terms of functionally enriched pathways in the tumor cell lines. A total of 64 (BT474-184A1) and 69 (SKBR3-184A1) proteins were uniquely identified that were differentially expressed by at least 1.5-fold. ResultsĪ proteomic workflow was used to identify proteins in two HER2 positive tumorigenic cell lines (BT474 and SKBR3) that were differentially expressed relative to a normal human mammary epithelial cell line (184A1). The objectives of this study were to quantify alterations in protein expression in two HER2+ cellular models of breast cancer and to infer differentially regulated signaling pathways in these models associated with the hallmarks of cancer. Yet how these genetic alterations translate into specific proteomic changes that underpin acquiring the hallmarks of cancer remains unresolved. A contemporary view of the cancer genome reveals extensive rearrangement compared to normal cells.

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